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Image Search Results
Journal: Chemistry & biology
Article Title: Specificity analysis-based identification of new methylation targets of the SET7/9 protein lysine methyltransferase.
doi: 10.1016/j.chembiol.2010.11.014
Figure Lengend Snippet: Figure 6. SET7/9 Is Inhibited by Phosphorylation of the Target Peptide or Protein (A) Relative activity of SET7/9 on CelluSpot array derived from H3 peptides containing different combinations of PTMs (Table S2). Phosphorylation of T3, S10, and T11 abolished SET7/9 activity. (B) Influence of phosphorylation of nonhistone substrates on SET7/9 methyla- tion activity. GST-tagged nonhistone substrates of SET7/9 were incubated with PKA. All domains except Cullin1 were phosphorylated (Figure S5). Then, phosphorylated and mock treated domains were methylated by SET7/9. A complete loss or strong reduction of SET7/9 activity was observed with ZDN8, IRF1, MINT, PPARBP, and MeCP2. See also Figure S6.
Article Snippet: Cloning, Expression, and Purification of Proteins The sequence encoding human A kinase anchor protein 6 (AKA6) (residues 485–776; NCBI accession number NP_004265.3), Bromodomain containing 1 (BRD1) (residues 681–891; NCBI accession number NP_055392.1), Centromeric protein C1 (CENPC1) (residues 279–474; NCBI accession number NP_001803.2), Dnmt1 (residues 622–780; NCBI accession number NP_001370.1), Methyl CpG binding protein 2 (MeCP2) (residues 201–486; NCBI accession number NP_004983.1), Msx2-interacting protein (MINT) (residues 1994–2281; NCBI accession number NP_055816.2), PPAR binding protein (PPARBP) (residues 847–1084; NCBI accession number NP_004765.2), Retinoblastoma binding protein 5 (RBBP5) (residues 323– 538; NCBI accession number NP_005048.2), Zinc finger DHHC domain containing 8 (ZDHHC8) (residues 227–400; NCBI accession number NP_037505.1), PRP4 pre-mRNA processing factor 4 homolog B (PRP4) (residues 93–396; NCBI accession number NP_789770.1) CDC2 related protein kinase 7 (CRKRS) (residues 2–339; NCBI accession number NP_057591.1), Cullin1 (residues 2–250; NCBI accession number NP_003583.2), TTK (residues 603–799; NCBI accession number NP_003309.2), and
Techniques: Phospho-proteomics, Activity Assay, Derivative Assay, Incubation, Methylation